primary human renal proximal tubule epithelial cells rptecs  (ATCC)

Journal: Frontiers in Molecular Biosciences

Article Title: Arsenic trioxide-induced acute kidney injury: OPA1- and Drp1-mediated mitochondrial dynamics imbalance, PINK1/Parkin-dependent mitophagy, and Chuanhuang Fang III

doi: 10.3389/fmolb.2026.1778855

Figure Lengend Snippet: CHF protects against AI-AKI by modulating renal tubular epithelial cell MQC in rats. (A, B) Mitochondrial membrane potential (Δψm) was detected by JC-1 staining. (C–F) OPA1 and Drp1 expression was assessed by immunofluorescence (scale bar = 40 µm) in rat kidney tissues. (G, H) Flow cytometry results of mitochondria ROS in rat kidney tissues. (I) Western blotting analysis of OPA1, Drp1, BNIP3, NIX, Parkin, PINK1, LC3B, Bcl-2 and BAX. (p <0.05, *p <0.01 vs CON or ATO or NAC, n=3).

Article Snippet: Human renal proximal tubular epithelial cells (HK-2, American Type Culture Collection, Manassas, VA, United States) were cultured in DMEM/F12 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques: Membrane, Staining, Expressing, Immunofluorescence, Flow Cytometry, Western Blot

Journal: Frontiers in Molecular Biosciences

Article Title: Arsenic trioxide-induced acute kidney injury: OPA1- and Drp1-mediated mitochondrial dynamics imbalance, PINK1/Parkin-dependent mitophagy, and Chuanhuang Fang III

doi: 10.3389/fmolb.2026.1778855

Figure Lengend Snippet: Mechanism diagram of CHF against AI-AKI by regulating the OPA1- and Drp1-mediated mitochondrial dynamics imbalance and mitophagy in renal tubular epithelial cells.

Article Snippet: Human renal proximal tubular epithelial cells (HK-2, American Type Culture Collection, Manassas, VA, United States) were cultured in DMEM/F12 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 .

Techniques:

Journal: International Journal of Biological Sciences

Article Title: Gastrodin alleviates high fructose-induced podocyte mitochondria-mediated apoptosis by inhibiting NLRP6 to facilitate TRIM7-triggered Bok mRNA degradation

doi: 10.7150/ijbs.120307

Figure Lengend Snippet: NLRP6 expression is increased in high fructose-induced glomerular podocyte injury in mice (A) Nlrp 6 and Nlrp12 mRNA levels in WT mouse glomeruli, ( n = 6). (B) Nlrp 6 and Nlrp12 mRNA levels in WT mouse glomeruli with or without HFrD, ( n = 4-6). (C) The representative micrographs of RNAscope ( Nlrp6 mRNA, cyan) and IF (Podocin, green; Synaptopodin, red) co-labeled the podocytes of mouse glomeruli. Scale: 25 μm. (D) Western blot detection of NLRP6 in WT mouse glomeruli with or without HFrD, (n = 7). (E) Representative IF images of NLRP6 (green) and Synaptopodin (red) co-labeled the podocytes of mouse glomeruli. Scale: 30 μm. (F) Western blot detection of NLRP6 in kidney cells, ( n = 4). (G) Western blot detection of NLRP6 in kidney cells exposed to 5 mM fructose, ( n = 4). (H) Western blot detection of NLRP6 in podocytes (HPCs, MPC5, PMPCs) exposed to 5 mM fructose or not, ( n = 4-6). (I) Representative IF images of NLRP6 (green) in podocytes (HPCs, MPC5, PMPC) exposed to 5 mM fructose or not. HPCs, Scale: 20 μm; MPC5, Scale: 20 μm; PMPC, Scale: 20 μm. NC: normal chow; HFrD: high fructose diet; HPCs: human podocytes; MPC5: mouse podocyte clone-5 cell; HGMC: human glomerular mesangial cell; PMGEC: primary mouse glomerular endothelial cell; HK-2: human proximal tubule epithelial cell; PMPC: primary mouse podocyte. Data are expressed as the mean ± SEM. ** P < 0.01, *** P < 0.001.

Article Snippet: Human proximal tubule epithelial cells (HK-2) were obtained from Cell Bank of Chinese Academy of Science, supplied by ATCC in Wuhan University, and maintained in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 0.1 mg/mL streptomycin at 37 °C with 5% CO 2 .

Techniques: Expressing, RNAscope, Labeling, Western Blot